RightOnTM Cancer Sequencing Enrichment Technology
Designed with advanced library prep and enrichment technologies, RightOnTM Cancer Sequencing Kits feature exceptional coverage and specificity, as well as high sensitivity for superior performances.
Figure 1 shows the percent of target bases covered as sequencing increases at four sequencing depths. With 5 – 6 GB sequencing, RightOnTM can achieve 100x minimum coverage for >98% of targeted bases.
Figure 1 Coverage per targeted base comparison across four sequencing depths. The RightOnTM Cancer Sequencing enriched samples were sequenced using Illumina 2x50bp and 2x100bp reads to provide the coverage depths.
Figure 2 illustrates the exceptional high specificity exhibited by RightOnTM Cancer Sequencing, where only the targeted regions are enriched.
Figure 2 Specificity of RightOnTM cancer panel is visualized from IGV view of one target gene. Only the desired targeted regions are enriched.
|Coverage for low Input DNA Samples|
|Sample Name||Blood DNA 1||Blood DNA 2||FFPE 1||FFPE 2|
|DNA Input Quantity||50 ng||50 ng||50 ng||50 ng|
|Sequencing Data||3.1 G||9.1 G||3.0 G||5.9 G||3.0 G||7.3 G||3.3 G||7.0 G|
|1x Min. Coverage||99.64%||99.97%||99.64%||99.96%||99.77%||99.95%||99.75%||99.95%|
|50x Min. Coverage||98.33%||99.62%||98.33%||99.45%||98.51%||99.57%||98.77%||99.54%|
|100x Min. Coverage||6.14%||99.09%||96.38%||98.49%||95.44%||98.93%||96.92%||98.89%|
Table 1 shows target bases covered for low input (50ng) DNA samples as sequencing increases. RightOnTM can achieve 100x minimum coverage for >98% of targeted bases with as low as 5 – 6 GB sequencing.
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