DNA Sequencing - Sample
(Effective Date: Monday, October 14, 2002)
To submit samples, you can either premix the template and the
primer, or send them separately. However, please do not premix for the
first a few times, so that we can check your template and calibrate the
concentrations (free for the first 3 times for new customers). The followings are
the conditions for both premixing and non-premixing sample preparation. When
possible, please use 8-strip PCR tubes for <96 samples and primers, and use
96-well plates for > 96 samples:
I. If you are not premixing the samples, please send according to the
1. Non-premix Template (in water):
|PCR products: concentration >10ng/ul, please send >10ul and let us
know the concentration and the length of the PCR product.|
|Plasmids: concentration >50ng/ul (100ng/ul is preferred), please send > 10 ul each.|
2. Non-premix Primer (in water): concentration 3 uM, please send > 10 ul
each, and have enough for all the samples.
II. If you premix the template with the primer:
1. Premix Template (in water):
|100-200bp 2-3 ng|
|200-500bp 6-8 ng|
|500-1000bp 10-15 ng|
|1000-2000bp 20-30 ng|
|>2000bp 60-100 ng|
|Double-stranded: (plasmids) 500ng|
2. Premix Primer (in water): 8 pmole
3. Premix Total volume (in water): 15 ul.
For Non-premix samples, we have the following additional services available.
We can prepare templates at $10/each (miniprep for plasmids, or purification of PCR
products). If you are not sure about the template concentration, we can check it
for you at $2/each. For templates with low, but reasonable concentration, we may
also concentrate the sample before the run at $5/each.
have M13 forward (-20); M13 forward (-40); M13 reverse; T7, T7 reverse, T3; and SP6
available as common primers. However, for the less popular common primers (M13 reverse -40; Lambda GT11 forward and reverse; SK; KS; 5' or 3'
pKK; pET; etc.),
if you anticipate that you'll use them a lot and would like us to stock them for
your non-premixed samples, we can do that after confirming the primer sequences with you.